Serodetection of Antibodies for Mycobacterium Avium Sub-Species Paratuberculosis in Sheep along with Its Associated Risk Factors in District Rahim Yar Khan, Punjab, Pakistan

  • Muhammad Arif Rizwan
  • Waseem Yaqub
  • Muhammad Kaleem
  • Muhammad Kashif Iqbal
  • Amjad Islam Aqib
  • Mahar Abdul Qudus
  • Saqib Umer
Keywords: ELISA, mycobacterium avium subsp. paratuberculosis, risk factors, sheep.


During this study identification of the antibodies of Mycobacterium avium subspecies paratuberculosis (MAP) prevalence and risk factors associated with paratuberculosis in sheep population of District Rahim Yar Khan, Punjab, Pakistan was carried out. For this purpose, 100 blood samples were randomly collected from slaughtered and marketed animals (irrespective of breed, age and sex) in district Rahim Yar Khan, Pakistan. Then samples were analyzed through ELISA for detection of MAP. Out of hundred animals 5.0 % were observed to be serological positive. In comparison to non-infected animals (control), the decreased in the total serum proteins (P<0.05) were noticed in the MAP infected animals. Furthermore, it was found that some risk factors can be responsible as a resource of spreading the paratuberculosis such as 92% malnutrition, 77% poor sanitation, 85% combine housing, 87% open grazing and 29% tick infestation. It was concluded that 5.0 % prevalence was occurred in the sheep population and might be some risk factors were also involved to spread the disease.