Coproduction of Protease, Amylase and Lipase from Fruit and Vegetable Waste Using Aspergillus Niger Fcc-Asq-06

  • Hadia Akber Samoo
  • Jagarwanti Maheshwari
  • Imrana Khushk
  • Chaudhary Haider Ali
  • Abdul Nabi Mirjatt
  • Abdul Sattar Qureshi
Keywords: Multi-enzyme production, Aspergillus niger FCC-ASQ-06, fruit and vegetable waste, solid state fermentation


In this study, multi-enzyme (amylase, protease and lipase) production from Aspergillus niger FCC-ASQ-06 was evaluated. Different fruit (banana peel, orange peel, lemon) and vegetable (pea peel) waste were tested as carbon sources. Maximal amylase and lipase production were 4.43 U/g DM and 6.44 U/g DM when banana peel was used as carbon source whereas highest protease production of 3.66 U/ g DM was noted when lemon peel was used as carbon source. Production rate further increased with fermentation time and maximum enzyme activities were noted after 144 h fermentation time when banana peel was used as carbon source. In the next step, effect of nitrogen sources on different enzyme production were checked. Maximal protease, amylase and lipase production were 12.4 U/ g DM, 13.4 U/g DM and 15.6 U/g DM, respectively when peptone and yeast extract were used as nitrogen source. Maximal enzyme production was noted from pH 6-8. Fermentation medium cost is always technical barrier in industrial scale processes for bioethanol, organic acids and enzyme production (lipase, amylase and protease). These enzymes have applications in various industries including food industry, saccharification of starchy materials, detergent and textile industry. This is why, there is need to find cost effective medium for multienzyme production. Utilization of fruit and vegetable waste in biotechnology industry will certainly reduce commodity production cost and solve pollution problems.