In Vivo Evaluation of Ginger Stem Extract as Repellent against Hyalomma Anatolicum Tick

Abstract

: The study was carried out during the year 2013-14 to evaluate the in vivo efficacy of Ginger Stem Extract as Repellent Against Hyalomma anatolicum Tick. For this purpose Hyalomma anatolicum engorged ticks were collected from cattle and buffalo at Livestock Experimental Farm, Sindh Agriculture University, Tandojam. The results shows that In experiment-1; in vivo repellency of ginger against Hyalomma anatolicum shows average 100%, 80%, 70% and 60% reppelency, in experiment-2; in vivo repellency of 90%, 70%, 60% and 50%, in experiment-3; in vivo repellency of 90%, 80%, 60% and 40%, in experiment-4; in vivo repellency of 80%, 70%, 60% and 40%, in experiment-5; in vivo repellency of 90%, 80%, 60% and 40% at 5%, 2.5%, 1% and 0.5% concentration of ginger extract. Overall, repellency was 76%, 64%, 50% and 32% under invivo conditions at 5%, 2.5%, 1% and 0.5% concentration of ginger extract. In experiment-1; 70%, 70%, 50% and 30% mortality of ticks was examined under in vivo conditions. In experiment-2;50%, 100%, 70% and 30% mortality of ticks under in vivo conditions at 5%, 2.5%, 1% and 0.5% concentration. In experiment-3; average 40%, 40%, 50% and 40% mortality of ticks under in vivo conditions at 5%, 2.5%, 1% and 0.5% concentration. In experiment-4; overall, 40%, 40%, 30% and 20% mortality of ticks under in vivo condition at 5%, 2.5%, 1% and 0.5% concentration. In experiment-5; overall, 40%, 40%, 30% and 20% mortality of ticks under in vivo condition at 5%, 2.5%, 1% and 0.5% concentration. Overall, 66%, 52%, 42% and 26% mortality of ticks under in vivo condition at 5%, 2.5%, 1% and 0.5% concentration. Zero mortality was seen in control group in all experiments. The calculations of LC 50 and LC 90 of ginger stem extract on Hyallomma anatolicum ticks shows that 33.75% ticks were died at lethal dose of 5% concentration and 32.25 % ticks were died at 2.5% concentration of ginger stem extract. From the present study it was concluded that in vivo efficacy of ginger extract against Hyalomma anatolicum ticks was agreeable