Main Article Content
Pakistan has long history to use medicinal plants as an alternative medicine. Excessive intake of plant based bioactive compounds some time lead to cellular damages. In this regard evaluation of non-cytotoxic dose to human can initially be tested in plant based assay. Therefore, current work mainly focuses phytotoxic effects of different of radish seeds (R.S.) Rhizome of Land-calotrops root (L.R.) that were partitioned in germination inhibition and mitotic index (M.I.) in chickpea seeds. The 20 seeds per treatment were incubated for 15, 30, 45 and 60 minutes in filtered aqueous extracts (1, 3 and 5%). Un-treated seeds were used as negative control and 0.2% Ethylmethane sulphonate treated seeds as positive control. Chickpea seed germination and mitotic index exhibited highly significant variations at p<0.01 (LSD) for most concentrations and their incubations for both tested plants. The inhibitory effects on germination were concentration and time dependent while mitotic index was mostly random for majority of treatment time and concentrations. Over all concentrations of R.S were less phytotoxic than L.R. as compare to +ve control.