Prevalence and Antimicrobial Resistance of E.Coli and Salmonella isolated from Poultry litter in District Shaheed Benazir Abad

Abstract

Poultry industry provides rich and cheap resources of protein requirements to the people in the form of chicken meat and eggs. The purpose of this study was to assess the sanitation conditions of different poultry farms in District Shaheed Benazir Abad of Sindh. A total of 100 poultry litter samples from four talukas i.e., Sakrand, Nawab Shah, Qazi Ahmed, and Daur were collected and transported to Central Veterinary Diagnostic Laboratory Tandojam for isolation and identification of microorganisms. The overall results showed that (84%) samples were found positive for E. coli and Salmonella species. Higher prevalence of E.coli 17(68%) was observed in Sakrand, while lower 12(48%) was observed in Dour. High prevalenceof Salmonella 9(36%) was observed in Nawab shah, while lower 5(20%) was observed in dour.  The results of antimicrobial sensitivity indicated that E.colis pecies were resistant to Tetracycline, Ciprofloxacin, Sulphafurazole and Oxacillin. However, E. coli species were sensitive to Erythromycin, Amikacin and Gentamycin. The isolated Salmonella species have shown resistance to Tetracycline, Ciprofloxacin and Sulphafurazole. While, Salmonella species were highly sensitive against the Amoxicillin, Erythromycin and Gentamycin. In conclusion, higher prevalence of E. coli was observed in Sakrand, while higher prevalence of Salmonella was observed in Nawab shah and lower prevalence of E.coli and Salmonella was observed in Dour. Whereas, the Tetracycline, Ciprofloxacin, Sulphafurazolewere highly resistant to E.coli and Salmonella.