Evaluation of Quality and Quantity of Tobramycin Sulfate from Different Pharmaceutical Dosage Forms in Various Brands Available in Pakistan.

Abstract

A qualitative and quantitative analysis of parenteral preparations and ophthalmic preparations were performed. In qualitative analysis the color, clarity, sterility, pH and filled volume tests were performed and for quantitative analysis a new spectrophotometric method was developed to analyze the pure form and commercial brands. In this method Tobramycin was reacted with vanillin a shiff’s base was produced. The yellow colored derivative showed the absorbance at 390 nm molar absorptivity of 1.141x104 L/mole/cm and obeyed the Beer’s law at the concentration of 03-15µg ml-1 against the absorbance with 0.9998 as value of coefficient of determination (r2). The effect of analytical parameters like optimization of ph, heating time and volume of reagent were also observed. The derivative was stable up to 72 hours. The qualitative parameters mentioned above were within specified range as mentioned in USP. The percentage of recovery was 103-105% from parenteral and 103.6-105% from ophthalmic preparations