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Gender Biasness is one of the most important topics in the field of human resource management especially in the categories of under developed countries and least developed countries of South East Asia like Pakistan where most of the professions are dominated by males. In these countries females are treated as home manager and their role is to support their spouse through taking responsibilities home and child care etc. On the other hand females are constantly ignoring these myths as they are found to be keenly interested in interest is having profound professional careers. In fact research also highlighted that there is a considerable increase in the number of female workforce during last decade but it has been also highlighted that most of the times females are opting those professions which are coherent with their attitudes and personalities like education and they have least acceptance in male dominated professions. Moreover their representation on top level positions is also in far and few between as there are several factors which are hindering in their way of career excellence. Thus there is a systematic need of investigation regarding those factors which are creating hindrance in the female career paths. Hence the aim of this research is to figure out those factors which are creating hindrance for female careers in the context of Pakistan and through combining literature and different research constructs a comprehensive research model has been develop to compare barriers associated with female careers with respect to different industries do to determine the impact of these barriers for each industry. Hence it is legitimate to believe that this research is pervasive in nature and will help managers in devising organizational policies more effectively. Therefore for the statistical part second order CFA and Path Analysis has been indulged for validating the research model and through the of structural equation modeling so to predict the impact of different constructs e.g. familial, cultural and organizational on female careers operating in higher education, banking, construction and production sector of Karachi. Thus through this research theory building might be enhanced in the context of Asian and under developed countries and researchers might able to perform optimized level of research in the betterment of society.