Main Article Content
A simple, precise, sensitive, economic, reproducible and validated RP-HPLC method was developed for the quantitative determination of Rifampicin in bulk and tablet dosage form. Analysis was carried out using acetonitrile: 0.1% orthophosphoric acid (80:20) mobile phase at 0.4 mL/min flow rate and Zorbax Eclipse Plus C18 columnas stationary phase with detection wavelength of 480 nm. The retention time was found to be 4.7 min. The method showed good linearity in the range of 1.95-250 μg/mL with correlation coefficient of 0.9996. Limit of detection and limit of quantification was found to be 0.607 and 1.841μg/mL, respectively. The method was validated for specificity, linearity, precision, accuracy, and limit of detection and quantification following ICH guidelines.