ANALYTICAL IVESTIGATIONS OF NEWLY PREPARED COMPLEX OF ALANINE AND 8-HYDROXYQUINOLINE USING MULTIPLE SPECTROSCOPIC METHODS

Abstract

Alinine occurs in body fluids in considerable amounts and is involved in various metablolic functions, such as together with Glycine it makes a considerable fraction of the amino nitrogen in human plasma The use of 8-hydroxyquinoline (Oxine) for identification and analysis of amino acids on TLC plates and flurometerically in nano mole quantity has already been reported by new methods accomplished in our laboratories (1-3). In present work Alanine-Oxine complex has been repared and its stoichiometric investigations has been carried out involving Spectrofluorometric, IR, NMR, TGA-DTA, Mass spectrometoric techniques. The results revealed that alanine was stoichiomexicallyboun with 8-hydroxquinoline molecules in 1:2 ratio for amino axau to reagent Determination of offlouresconi complex of alanine-oxane in UV region using TLC scanner and exciation and emission wavelengt by spectrofluorometar were found to be of immense importar a foextimation of amiro acids down to nano mole range.