Comparative Study of Interleukin 28B Gene in Genotype 3 Chronic Hepatitis C Patients Before and After Treated With Interferon/Pegylated in Sindh

Abstract

Chronic hepatitis C (HCV) caused by hepatitis C (HCV) virus, approximately estimated 170-200 million individuals in the world including Pakistan having 10 million HCV infected . The effective available treatment option is a combination of Pegylated interferon (PegIFN) and Ribavirin (RBV) . Recently Genome wide association studies (GAWAS)supporting two single nucleotide polymorphism SNPs adjacent interleukin 28B gene associated with spontaneous and treatment induced clearance of HCV patients. The aim of the present study was to analysis the impact of response associated interleukin 28B(IL28B) single nucleotide polymorphism (SNPs) rs12979860, rs8099917 in different treatment interval with one month (RVR) and after end of treatment (SVR) with Pegylated Interferon(PEG IFN) and Ribavirin in Hepatitis C genotype 3 patients. The present study enrolled 200 HCV RNA positive genotype 3 patients having both SNPs (rs12979860, rs8099917) were analyzed by Real Time Polymerase Chain Reaction (RT-PCR). The frequency distribution of IL28B genotypes were investigated by SNIPs (rs12979860 and rs8099917) in HCV RNA positive genotype 3 patients in Pakistani population.