Electrophoretic characterization of possible allergens in population of Hyderabad, Sindh.

Abstract

Wheat is the most consumable food in the diet worldwide especially in Pakistan and important dietary source of proteins. Some proteins of wheat may be harmful for some people because it triggers the immune response of the person. The present study was an effort to explore the prevalence of food allergy in adult and children in population of Hyderabad Sindh. The participants included in our study are in age ranged 04 - 40 yearsin which 03 children are in infant age while other included different age groups and gender. Ingestion of wheat products in the children can escort the variety of problems mainly abdominal pain and respiratory symptoms. The SDS-PAGE pattern of the selected allergic individual shows at least 02 possible allergens present in all the patients with allergic symptoms asthma, vomiting, urticaria, wheezing, gastrointestinal symptoms, abdominal pain, and respiratory symptoms. Serum of these selected allergic patients mainly contains 15 kDa, 17 kDa, 20 kDa, 47 kDa and 77 kDa major allergenic proteins. The allergic response of individuals was investigated by ELISA, The antibody (IgE) response confirmed that level of IgE of these individual are very high ranged varied from 300-1200 IU/ml.