Phenotypic determination of specific virulence factors in Staphylococcus aureus circulating at Hyderabad, Pakistan

  • N. KUMARIKUMARI
  • S. JABEEN
  • U. SHAIKH
  • A. SHEIKH
  • N. SAEED
  • T. RAFAQAT
  • K. FATIMA
Keywords: Staphylococcus aureus, virulence factors, staphylokinase, lipase

Abstract

Understanding of virulence characteristics which help Staphylococcus aureus strains to survive in clinical and non-clinical settings is crucial for antibacterial strategies. This study is designed to investigate some phenotypic virulence traits if they relate S. aureus to defined environment. For this purpose fifty-three S. aureus cultures were isolated from clinical (n=22) and non-clinical (n=31) environments and processed for the determination of selected virulence factors i.e., Staphylokinase, lipase and haemolysins on specific media plates. The results showed that 39.6% (n=21) of total isolates were staphylokinase producers. Categorically the frequency of Staphylokinase producing isolates was higher among the clinical isolates (50%) than the non-clinical isolates (32.2%). Overall 81% (n=43) isolates were lipase producers. Unequivocally, the frequency of lipase production was higher among clinical isolates (86.3%) than the non-clinical isolates (77.4%). The haemolytic potential was observed in all tested S. aureus isolates. Beta-haemolytic pattern was most frequent observation among both clinical and non-clinical isolates. Interestingly, 81% of Staphylokinase producers showed beta-hemolytic pattern. In conclusion, the current data suggest that S. aureus in clinical setting are more virulent than non-clinical isolates circulating at Hyderabad region

Published
2020-06-23